SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
sequencing 16s rRNA Nullie RNA Sequencing 10 11-16-2016 07:53 AM
16S rRNA amplicon sequencing kit ChristmasSunflower Illumina/Solexa 3 09-06-2013 07:30 AM
Direct 16S amplicon sequencing? lorendarith Illumina/Solexa 2 03-04-2013 09:41 AM
16s rRNA gene sequencing(metagenomics) newBioinfo Sample Prep / Library Generation 0 03-26-2012 05:08 PM
16s rRNA gene sequencing(metagenomics) newBioinfo Illumina/Solexa 0 03-26-2012 02:24 PM

Reply
 
Thread Tools
Old 12-17-2015, 11:39 AM   #21
Brian Bushnell
Super Moderator
 
Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707
Default

Quote:
Originally Posted by fibar View Post
But what has happened in the field during the last year? Are there improved methods to deal with this problem?
In fact, there IS an improved method for dealing with noise and false-positives. BBMerge has a much lower false-positive merge rate than any other overlap-based read merger, including usearch.
Brian Bushnell is offline   Reply With Quote
Old 01-21-2016, 12:43 AM   #22
elizondas
Member
 
Location: oxford

Join Date: Nov 2013
Posts: 14
Default Primers and barcodes for V1-V2

Hello,

I was wondering whether any of you has sequenced using Illumina primers for V1-V2 region....Does anyone have a list of barcodes I could used? We need to focus on this region, since it is the best approach to identify sp within oral samples. ...

Many thanks
Elisa
elizondas is offline   Reply With Quote
Old 07-21-2016, 12:02 AM   #23
ymc
Senior Member
 
Location: Hong Kong

Join Date: Mar 2010
Posts: 497
Default

Suppose I can the sequence of the full 16S gene at high accuracy, which region(s) can give me the closest discriminating power to the whole gene sequence?
ymc is offline   Reply With Quote
Old 07-21-2016, 04:02 AM   #24
Brian Bushnell
Super Moderator
 
Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707
Default

I don't think that question's been answered. But, you might want to look at this paper, which compares V4 to full-length:

http://www.nature.com/ismej/journal/...j2015249a.html
Brian Bushnell is offline   Reply With Quote
Reply

Tags
16s rrna, miseq, v1-2 region, v4 region

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:23 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO