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Old 12-18-2017, 02:42 PM   #1
AlejandroRivera
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Location: Glasgow

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Default Multiplexing in Miseq

Hi all

Im new to the world of NGS and I want to know how many samples can I multiplex in one run using a Miseq sequencer, details are as follow:

Depth of sequencing 30X
Size of the regions I want to sequence is 10.5Mb
Sequencer is Miseq and I can use reagent kit 2X75 300 cycles v3 (3.8gb output)

Thanks

Alejandro
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Old 12-19-2017, 12:44 AM   #2
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Data Output of Sequencer divided by (target size * coverage) = number of samples.

12 samples in your case not accounting for off-target reads (phiX, enrichments etc.)
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Old 12-19-2017, 04:44 AM   #3
Bukowski
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https://support.illumina.com/downloa...alculator.html
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