RNA sequencing (RNA-seq) has become a central tool with wide-ranging research and clinical applications. However, accurate resolution of gene expression is confounded by the sheer size and complexity of the transcriptome and further exacerbated by technical variables during library preparation, sequencing and bioinformatic analysis.
We have developed a suite of synthetic RNA isoforms (termed ‘sequins’) that align to artificial gene loci encoded within an accompanying in silico chromosome. Sequins can be spiked into a natural RNA sample and constitute internal controls for evaluation of the RNA-seq workflow, including library preparation, sequencing, split-read alignment, transcript assembly, gene expression, alternative splicing and fusion gene detection.
Please see our recent Nature Methods publication for a full description of sequins for RNA-seq.
This thread provides a forum for current and future sequin users to discuss any aspect of this emerging technology.
You can also watch this short video, which provides an introduction to sequins or visit http://www.sequin.xyz/ for more details.
Cheers
Simon Hardwick (on behalf of the Sequin team)
We have developed a suite of synthetic RNA isoforms (termed ‘sequins’) that align to artificial gene loci encoded within an accompanying in silico chromosome. Sequins can be spiked into a natural RNA sample and constitute internal controls for evaluation of the RNA-seq workflow, including library preparation, sequencing, split-read alignment, transcript assembly, gene expression, alternative splicing and fusion gene detection.
Please see our recent Nature Methods publication for a full description of sequins for RNA-seq.
This thread provides a forum for current and future sequin users to discuss any aspect of this emerging technology.
You can also watch this short video, which provides an introduction to sequins or visit http://www.sequin.xyz/ for more details.
Cheers
Simon Hardwick (on behalf of the Sequin team)
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