Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Errors loading particular vcf files into IGV but not others

    Hi,

    (would've posted this in the google group but it's not letting me for some reason, so I thought I'd try SEQanswers instead)

    IGV version: 2.2

    What I did:
    - File -> Load from file -> indels.vcf

    What I expected to happen:
    Loading of indel variations into track of IGV browser

    What actually happened:
    Error loading /filepath/Indels.vcf: Line: chr1 821351 . TGCTGTGGTT----- TAGGGCTGATATGGA 357 PASS DP=55 GT:GQ 0/1:357

    Thought it might be a problem with the index for this vcf file, so reindexed it with igvtools and the following error came up whilst running igvtools:
    Error: Line: chr1 821351 . TGCTGTGGTT----- TAGGGCTGATATGGA 357 PASS DP=55 GT:GQ 0/1:357

    This is an Illumina generated file, I have other vcf files from other individual vcf files from the same batch that load into IGV fine (and some that have similar errors including: Error: The provided VCF file is malformed at approximately line number 110: Unparsable vcf record with allele TGCTGTGGTT----- when using igvtools to index).

    This file and others have loaded fine into previous versions of IGV. I just haven't accessed it for a while.

    Any thoughts on what's going wrong?

    Cheers,

    Nick
    Nicholas Blackburn | PhD Candidate
    Tasmanian Inherited Cancer Centre
    Menzies Research Institute Tasmania
    Hobart TAS 7000 | www.menzies.utas.edu.au

  • #2
    Maybe it is an old VCF format? IGV 2.2 read VCF version 4.0.

    Comment


    • #3
      Nice thinking TiborNagy, but they're all VCF versions 4.1.
      Nicholas Blackburn | PhD Candidate
      Tasmanian Inherited Cancer Centre
      Menzies Research Institute Tasmania
      Hobart TAS 7000 | www.menzies.utas.edu.au

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        Today, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 07:17 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-02-2024, 08:06 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-30-2024, 12:17 PM
      0 responses
      20 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-29-2024, 10:49 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Working...
      X