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You will have to forgive me but I know very little about Linux and python, I have installed MIRA in order to assemble 454 reads and Illumina reads from a couple of bacterial genomes. However, before I can run MIRA with 454 reads I need to remove the adaptor sequence, so they suggest using sff_extract. I went to the website http://bioinf.comav.upv.es/sff_extract/index.html, but when I click on the download link it just gives me the line code for the program. I do not know what to do with this code in order to get the program loaded, so that I can trim my sequences for MIRA. I also do not have access to the sfftools that come with the 454/Roche software package as the sequencing was outsourced to a company and they claim not to know what I'm talking about when I ask for them. Any help would be very much appreciated, I appreciated everyone's help answering my stupid questions. Thank you in advance.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Today, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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