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Old 05-03-2011, 08:00 AM   #1
Location: Boston

Join Date: Apr 2011
Posts: 18
Default DIY illumina adapters

Hi All:
Trying to determine whether I can get away with making my own adapters for the HiSEQ 2000.
I've got a library of small DNA (125nt) fragments, and would like to sample the diversity of this library pre- and post-selections.

I get it that there is that funky fork with a Phosphorothioate Bond...and that the Tm of the sequencing primer is in the high 70s. Has anyone built their own adapters, or should I just buy the genomic DNA prep kit...?

Also, I am not compiling huge contigs, but rather, assessing the diversity of the library through selections...what, other than the QC descriptions, do I need to assess this diversity? Can someone point me towards the PERL scripts that would process these data into counted bins of reads?
ZAAB is offline   Reply With Quote

adapter, counting, hiseq, illumina, individual

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