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  • Training Augustus with RNA seq data

    Hello all,
    I am trying to annotate a genome (476 Mb ; 8,000 scaffolds).
    I plan to use the Maker pipeline and before that, I need to train Augustus.
    I have plenty of RNA seq data that I mapped and assembled using tophat and cufflinks.
    I read many tutorials for training Augustus but none is doing the same and I actually don't understand how I can do that... I am confused with many things, it is not clear at all...
    Is there a kind person to explain me the pipeline of training Augustus using RNA seq data ?
    Thanks a lot !
    Muriel

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