Hey all, I'm looking to sequence human exons across 189 genes totalling about 700kb. Is there anyone who's tried both target capture and amplicon sequencing who could tell me which would be better for this?
If I do go for amplicon sequencing, is there anything weird I should expect in the results? Does the non-physical shearing used by Haloplex etc. give you weird fragment sizes or loads of false PCR duplicates, or is that mostly not a problem?
If I do go for amplicon sequencing, is there anything weird I should expect in the results? Does the non-physical shearing used by Haloplex etc. give you weird fragment sizes or loads of false PCR duplicates, or is that mostly not a problem?
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