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Old 01-10-2017, 11:20 AM   #1
morning latte
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Default MinION sequencing kit

Hello experts,

When I am reading some publications using MinION, people used kits named MAP-003 Kits or MAP- 004 Kits for library generation. But when I look at the ONT website, I see three kits, Rapid Sequencing Kit 2, Ligation Sequencing Kit 1D (R9.4), and Ligation Sequencing kit 2D (R9.4)? What are the MAP-003 Kits or MAP- 004 Kits? I am planning to use MinION for viral genomes and not sure which kit I should purchase. Thank you for the help in advance.
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Old 01-10-2017, 12:55 PM   #2
nanos
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The MAP*** kits are outdated versions and are no longer available.
The selection of kit depends on your sample type:
RAD - rapid kit tagmenting the template DNA
Lig1D - Ligation of adaptors sequenicing 1 strand (1D)
Lig2D - Ligation of adaptor + hairpin adaptor sequencing both strands (2D - results in highter seq-quality)

The old MAP kits were most likely 2D ligation kits. By know I would guess the 1D sequencing provides same accuracy than the old MAP-2D kits but yield more output.
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Old 01-10-2017, 01:12 PM   #3
morning latte
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Thanks nanos!
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Old 01-10-2017, 01:47 PM   #4
gringer
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Quote:
Originally Posted by nanos View Post
The old MAP kits were most likely 2D ligation kits. By know I would guess the 1D sequencing provides same accuracy than the old MAP-2D kits but yield more output.
Yes, that's right. To further add to the confusion, ONT will be shortly bringing out a double-1D technology shortly that excludes the hairpin but produces a 2D consensus sequence.
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