I use cuffdiff to detect differential expressed genes in two samples, since each has 2 replicates, so i use commas to separate replicates.
It gives empty results;
But if I run:
It works fine.
I have no idea why the replicates doesn't work.
Code:
cuffdiff -m 40 genes.gtf /60PO/accepted_hits.bam , /6124/accepted_hits.bam /3584/accepted_hits.bam , /4189/accepted_hits.bam
But if I run:
Code:
cuffdiff -m 40 genes.gtf /60PO/accepted_hits.bam /3584/accepted_hits.bam
I have no idea why the replicates doesn't work.
Comment