Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • ERANGE: runRNAPairedAnalysis.sh with error at the rnafarPairs.py step

    Hi
    I am analyzing one PE lane with files ‘s_3_1_sequence.txt’ and ‘s_3_2_sequence.txt’, which I mapped with Bowtie and then used to create s3.rds.
    I got the error below when I run runRNAPairedAnalysis.sh
    Thanks for the help
    Joseph


    .....
    /proj/genome/commoncode/rnafarPairs.py: version 3.5
    caching ....
    dataset s3.rds
    metadata:
    bowtie_mapped True
    dataType RNA
    paired True
    rdsVersion 1.1
    readsize 34
    user J.Dhahbi
    7118451 unique reads, 590197 spliced reads and 3588212 multireads
    default cache size is 1000000 pages
    found index
    chr1
    Traceback (most recent call last):
    File "/proj/genome/commoncode/rnafarPairs.py", line 78, in <module>
    uniqDict = RDS.getReadsDict(fullChrom=True, chrom=achrom, noSense=True, withFlag=True, withPairID=True, doUniqs=True, readIDDict=True)
    File "/proj/genome/commoncode/commoncode.py", line 1021, in getReadsDict
    (theReadID, pairID) = readID.split('/')
    ValueError: unpack list of wrong size
    /proj/genome/commoncode/normalizeExpandedExonic.py: version 5.4
    human cached
    caching ....

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
18 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
22 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Working...
X