Hi all,
I've got RNA-seq paired-end Illumina MiSeq data. I'm stuck with which read now represents the forward strand, and which one the reverse complement strand. When mapping, which read (R1/R2) should map (equals part of) to the 5' -> 3' reference sequence? And which one should map to its reverse complement?
[Header]
IEMFileVersion,4
Experiment Name,sampleA
Date,12/10/2013
Workflow,GenerateFASTQ
Application,RNA-Seq
Assay,TruSeq LT
Description,
Chemistry,Default
I've got RNA-seq paired-end Illumina MiSeq data. I'm stuck with which read now represents the forward strand, and which one the reverse complement strand. When mapping, which read (R1/R2) should map (equals part of) to the 5' -> 3' reference sequence? And which one should map to its reverse complement?
[Header]
IEMFileVersion,4
Experiment Name,sampleA
Date,12/10/2013
Workflow,GenerateFASTQ
Application,RNA-Seq
Assay,TruSeq LT
Description,
Chemistry,Default
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