Hi Everyone,
Nice to meet you guys here!
I'm doing ChIP-Seq using HiSeq 1500 on SR 50 cycles, multiplexed condition.
And I got a funny curve in my FastQC report on Per sequence GC content and Sequence Duplication Levels as well as overrepresented sequence.
Anyone please give a help
1) Why my Per sequence GC content curve got a horn?
2) How come such high sequence duplication level like 71.09% ? Due to overrepresented sequence?
3) As attached BioAnalyzer, there's totally absence of adapter, why still overrepresented sequence like Index 7?
Library prep condition: 12cycles PCR amplification
Cheers!
kb
Nice to meet you guys here!
I'm doing ChIP-Seq using HiSeq 1500 on SR 50 cycles, multiplexed condition.
And I got a funny curve in my FastQC report on Per sequence GC content and Sequence Duplication Levels as well as overrepresented sequence.
Anyone please give a help
1) Why my Per sequence GC content curve got a horn?
2) How come such high sequence duplication level like 71.09% ? Due to overrepresented sequence?
3) As attached BioAnalyzer, there's totally absence of adapter, why still overrepresented sequence like Index 7?
Library prep condition: 12cycles PCR amplification
Cheers!
kb
Comment