Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • PubMed: Rapid High-throughput HLA Typing by Massively-parallel Pyrosequencing for Hig

    Syndicated from PubMed RSS Feeds

    Related Articles Rapid High-throughput HLA Typing by Massively-parallel Pyrosequencing for High-Resolution Allele Identification.

    Hum Immunol. 2009 Aug 22;

    Authors: Gabriel C, Danzer M, Hackl C, Kopal G, Hufnagl P, Hofer K, Polin H, Stabentheiner S, Pröll J

    Transplantation and notably haematopoietic stem cell transplantation require high resolution HLA typing and are therefore, because of the heterozygous genomic DNA samples, dependent on clonal analytical methods. High-resolution HLA typing is a necessity for accomplishing the best-possible histocompatibility match between donor and recipient, since mismatches strongly increase the risk of severe acute graft-versus-host disease. We describe the development and first application in a clinical setting of a novel, human leukocyte antigen sequence-based typing method by exploring the next-generation sequencing technology as provided by the Genome Sequencer FLX system (Roche / 454 Life Sciences, Branford, CT, USA). The developed system allows for ambiguity-free, high-throughput, high-resolution HLA-A and -B typing with the potential for automation. Primers and Genome Sequencer FLX specific adaptors were lengthened with donor-identifying barcode sequences to identify each of 8 Caucasian reference donors within one single multiplex sequencing run. Results, compared to normal SBT HLA typing show that with an average of 1000 reads per amplicon every patient was identified correctly. Furthermore, current investments for increased read lengths and fully automated molecular diagnostic software tools, using original GS-FLX data file formats, will enhance this novel HLA typing strategy in the near future.

    PMID: 19706315 [PubMed - as supplied by publisher]



    More...

Latest Articles

Collapse

  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM
  • seqadmin
    The Impact of AI in Genomic Medicine
    by seqadmin



    Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
    02-26-2024, 02:07 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-14-2024, 06:13 AM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-08-2024, 08:03 AM
0 responses
71 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-07-2024, 08:13 AM
0 responses
80 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-06-2024, 09:51 AM
0 responses
68 views
0 likes
Last Post seqadmin  
Working...
X