We generated multiple PCR products and have performed next generation sequencing (Illumina Hi Seq platform) to sequence 100-125 base pair PCR fragments. We need to find tool remove forward and reverse primers. Because size of fragment are different we cannot do clipping of N nucleotides.
I was wondering if there is primer pair removal tool or a program to get rid of the primers.
Thanks
-Shruti
I was wondering if there is primer pair removal tool or a program to get rid of the primers.
Thanks
-Shruti
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