Hello,
I am working with the 454 but I have problems with the PCR multiplex because I don't manage to avoid primer dimers. I carried out 2 consecutive PCR and the dimers reach to about 115-130 bp so they are not removed by Ampure purification. Do you have any other purification method that eliminate dimers or small inespecific amplicons of these sizes? or any recommendation to solve this problem?
Thanks in advanced,
I am working with the 454 but I have problems with the PCR multiplex because I don't manage to avoid primer dimers. I carried out 2 consecutive PCR and the dimers reach to about 115-130 bp so they are not removed by Ampure purification. Do you have any other purification method that eliminate dimers or small inespecific amplicons of these sizes? or any recommendation to solve this problem?
Thanks in advanced,
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