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Old 05-24-2013, 05:09 AM   #1
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Location: ZA

Join Date: May 2013
Posts: 1
Question AVA: No Variants To Load

We have received .sff files from a GS FLX 454 Titanium run of a single forward sequence amplicon. I have followed the AVA manual to create a reference, sample, amplicon, etc. and have made the necessary associations, and loaded in the SFF file. Blueprints, MIDs, etc. are all shown. The relevant "Active" entry is selected

However, after computing the results, I am always shown "No sample/reference data to align"" and "No variants to load". I have tried with a known variant, and without any variants specified. I have also tried with different blueprints selected.

Suggestions, please?

(Also posted here, for reference).

Last edited by SabreWolfy; 05-27-2013 at 02:39 AM.
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