Dear all,
I'd like to deplete tRNA and 5S rRNA from total RNA to clone and deep sequence primarily precursor miRNAs in eukaryotic cells. I'm assuming that pre-miRNA are at a very low concentration when compared to tRNA/5S so if these species are left in my sample preparation they will represent the vast majority of sequences. Any help and/or suggestions would greatly be appreciated.
Thanks.
I'd like to deplete tRNA and 5S rRNA from total RNA to clone and deep sequence primarily precursor miRNAs in eukaryotic cells. I'm assuming that pre-miRNA are at a very low concentration when compared to tRNA/5S so if these species are left in my sample preparation they will represent the vast majority of sequences. Any help and/or suggestions would greatly be appreciated.
Thanks.
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