Hi
Is it possible to mix equal molar of EZ-Tn5 transposase and the Nextera adapter to create a homebrew transposon?
It seems that the transposon in the Nextera kit contain 2 different adapter arms. But the conventional transposase and donor DNA complex should be 1 transposase + 1 DNA. So how did Epicentre prepare their transposon?
Is it possible to mix equal molar of EZ-Tn5 transposase and the Nextera adapter to create a homebrew transposon?
It seems that the transposon in the Nextera kit contain 2 different adapter arms. But the conventional transposase and donor DNA complex should be 1 transposase + 1 DNA. So how did Epicentre prepare their transposon?
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