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Old 10-21-2015, 05:08 AM   #1
jsvinco
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Location: Bordeaux

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Default Increase the number of cycle with a 50 cycles MiSeq Reagent Kit v2

Hello,

Is it possible to do a run of 60 cycles with a 50 cycles MiSeq Reagent Kit v2 ?
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Old 10-21-2015, 05:21 AM   #2
bunce
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Hi jsvinco,
Each kit is loaded with 25 additional cycles for indexing. So unless you need both indexing reads the answer is a 'yes'. If you need both indexing reads as well as the 50 cycles you might be pushing it - we have pushed the machine 5 cycles over the total cycle count (i.e 330 cycles on a 300 kit) and it behaved (although the machine complains at set-up). Other might have gone further?

Hope this helps - Cheers, Mike.
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Old 10-21-2015, 10:42 PM   #3
jsvinco
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Thank you for your help. It's very helpful to now that.

jsvinco
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