Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • visualizing RNAseq mapping to reference

    I'm using Geneious (for Windows), which has two RNA Seq mappers (Geneious RNA Mapper, and BBmap). I'm looking for introns in a set of individual gene sequences. Mapping results in Geneious are displayed in several ways, including a coverage track that shows introns plainly. However, there's no way to export this image. I can export output to SAM/BAM files, however. So, what vizualization tool can I use to produce an exportable graph that plots coverage vs nt position?


    EDIT: I found that Geneious allows exporting coverage data as .csv or .wig, so I'm good. But I'd still like to know how to generate plots or .csv files automatically for dozens of genes at once.
    Last edited by ssully; 03-04-2019, 03:52 PM.

  • #2
    You can use Integrated Genome Viewer (Broad Institute) to do batch analyses/exports of images. Details are included here.

    IGV-snapshot-automator is another thing you may be interested in.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    66 views
    0 likes
    Last Post seqadmin  
    Working...
    X