Hi all,
In the next month or so I will be illumina sequencing genomic dna and cDNA of 14 samples. The genome/transcriptome size I am working with is moderate (37Mb/18Mb) and I am going to try multiplexing gDNA and cDNA in the same lane using different adaptors. I am wondering 2 major things:
(1) Could anyone provide me with library preparation protocols for gDNA and cDNA? Are there any potential problems I should be aware of? What is the best way to QC samples?
(2) Has anyone tried multiplexing? What advice do you have?
Thank you in advance...your comments are greatly appreciated.
-John G
In the next month or so I will be illumina sequencing genomic dna and cDNA of 14 samples. The genome/transcriptome size I am working with is moderate (37Mb/18Mb) and I am going to try multiplexing gDNA and cDNA in the same lane using different adaptors. I am wondering 2 major things:
(1) Could anyone provide me with library preparation protocols for gDNA and cDNA? Are there any potential problems I should be aware of? What is the best way to QC samples?
(2) Has anyone tried multiplexing? What advice do you have?
Thank you in advance...your comments are greatly appreciated.
-John G
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