Hi everyone,
I designed several probes for highly polymorphic region to do target enrichment assay. In particular region, I will have around 200-300 different probes that are just different in one nucleotide to distinguish different alleles. I am unsure having probes with one or two nucleotides different will be affecting the enrichment assay to run in Miseq. Can anyone help me?
Thanks.
I designed several probes for highly polymorphic region to do target enrichment assay. In particular region, I will have around 200-300 different probes that are just different in one nucleotide to distinguish different alleles. I am unsure having probes with one or two nucleotides different will be affecting the enrichment assay to run in Miseq. Can anyone help me?
Thanks.
Comment