What volume of adapters do you get in the nextera XT kits?

NGSMicro · 11-07-2018, 06:01 AM

Hello,

At our lab we have a custom protocol for adding indices to our amplicons, using only 5uL of each index when using the "Nextera XT Index Kit (24 indexes, 96 samples)".

I want to update this to use the "IDT® for Illumina® Nextera™ DNA UD Indexes Set A (96 Indexes, 96 Samples)" kit, but I can't find out how much (approximately) you get in each kit for each pair of indices.

Has anyone bought this kit?

nucacidhunter · 11-08-2018, 02:47 AM

10 ul with some overage in each well which contains pool of two primers.

NGSMicro · 11-08-2018, 03:04 AM

Thank you, most appreciated.

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11-07-2018, 06:01 AM	#1
NGSMicro Junior Member Location: UK Join Date: Apr 2018 Posts: 8	What volume of adapters do you get in the nextera XT kits? Hello, At our lab we have a custom protocol for adding indices to our amplicons, using only 5uL of each index when using the "Nextera XT Index Kit (24 indexes, 96 samples)". I want to update this to use the "IDT® for Illumina® Nextera™ DNA UD Indexes Set A (96 Indexes, 96 Samples)" kit, but I can't find out how much (approximately) you get in each kit for each pair of indices. Has anyone bought this kit?

11-08-2018, 02:47 AM	#2
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	10 ul with some overage in each well which contains pool of two primers.

11-08-2018, 03:04 AM	#3
NGSMicro Junior Member Location: UK Join Date: Apr 2018 Posts: 8	Thank you, most appreciated.