Hi all,
I made 2 runs on Miseq using 300cycles (2X150). I used 2 different library preparation kits. One of them KAPA RNA Hyper prep and the second one Nextera dna flex library prep. The both libraries were normalized manually after I checked their quality by bioanalyzer. the first library was 10pM conc. and the other one 12pM (like the kit recommend). In both runs I got the same problem. The reads of R2 became low from beginning (the pic. attached will clarify the problem).
Plz if anyone has an explanation for this result, tell me.
Many thanks
I made 2 runs on Miseq using 300cycles (2X150). I used 2 different library preparation kits. One of them KAPA RNA Hyper prep and the second one Nextera dna flex library prep. The both libraries were normalized manually after I checked their quality by bioanalyzer. the first library was 10pM conc. and the other one 12pM (like the kit recommend). In both runs I got the same problem. The reads of R2 became low from beginning (the pic. attached will clarify the problem).
Plz if anyone has an explanation for this result, tell me.
Many thanks
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