Hello All
I am having trouble in amplifying small RNA library using Tru-Seq small RNA kit. I used 1 ug of Total RNA from grape leaf. I had checked the quality using Bioanalyzer (RIN was >8). I have followed the steps as mentioned in the manual precisely. I repeated twice with no result. Please help me trouble shoot this issue.
I am having trouble in amplifying small RNA library using Tru-Seq small RNA kit. I used 1 ug of Total RNA from grape leaf. I had checked the quality using Bioanalyzer (RIN was >8). I have followed the steps as mentioned in the manual precisely. I repeated twice with no result. Please help me trouble shoot this issue.
Comment