About Agilent DNA 1000 Kit

[amanbarc]

Dear All
I have stored Agilent DNA 1000 Kit (DNA Gel Matrix, DNA Dye Concentrate, DNA Markers, Spin Filters and DNA Ladder) in -20 °C, not in 4 °C. The actual storage temperature for all these materials are 4 °C. Initially, I have stored at 4 °C but 6-7 days before, I have shifted it to -20 °C. So now, I am afraid whether the kit is usable or not? Can anyone please help me to figure out this situation.

Regards
Aman

[nucacidhunter]

One way to work out if it is OK, would be to run a Chip with a DNA ladder or any other sample with known size and quantity. Analysing kit ladder and known sample would indicate if sizing and quantification are OK or not. If the quantification has been affected you still can use it for sizing.