I run this command:
hisat2 -p 40 -q -x Ene_index --sra-acc SRR13696274 --al alignedRNA_1 -S alignment1
and the output says:
4249510 reads; of these:
4249510 (100.00%) were unpaired; of these:
3194713 (75.18%) aligned 0 times
1045690 (24.61%) aligned exactly 1 time
9107 (0.21%) aligned >1 times
24.82% overall alignment rate
However, the produced alignedRNA_1 file is empty
Does anyone know what the problem may be?
Thanks
hisat2 -p 40 -q -x Ene_index --sra-acc SRR13696274 --al alignedRNA_1 -S alignment1
and the output says:
4249510 reads; of these:
4249510 (100.00%) were unpaired; of these:
3194713 (75.18%) aligned 0 times
1045690 (24.61%) aligned exactly 1 time
9107 (0.21%) aligned >1 times
24.82% overall alignment rate
However, the produced alignedRNA_1 file is empty
Does anyone know what the problem may be?
Thanks
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