I have got a RAD data set and assessed it. It seems the depth of RAD fragment vary differently, extremely different.
the reads(extract same reads) with depth 1 occupied 40%
but there are also others different reads have the depth from 2~100000.
I cannot image that a single reads have 100000 fold, is it a repeat in the genome? but are there so many repeat in genome?
the reads(extract same reads) with depth 1 occupied 40%
but there are also others different reads have the depth from 2~100000.
I cannot image that a single reads have 100000 fold, is it a repeat in the genome? but are there so many repeat in genome?
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