Hi,
I wanna analyze RNA-seq data of two time points (each one has a control and a knocked down sample) in order to find genes that are constitutionally down or up regulated. i was thinking of dividing the 4 samples into two groups Group1(Ctrl-time_point1, Ctrl-time_point2) and group2 (KD-time_point1, KD-time_point2) and apply a per gene t-test between two groups. Do you think this is a good idea? Any other suggestions??
I wanna analyze RNA-seq data of two time points (each one has a control and a knocked down sample) in order to find genes that are constitutionally down or up regulated. i was thinking of dividing the 4 samples into two groups Group1(Ctrl-time_point1, Ctrl-time_point2) and group2 (KD-time_point1, KD-time_point2) and apply a per gene t-test between two groups. Do you think this is a good idea? Any other suggestions??