cDNA normalization

Triticum · 09-15-2009, 12:56 AM

hi!
is it possible to do a cDNA-normalization prior to the sequencing with solid? the solid-protocol does not mention normalization, but i think it would be very useful for me. i´m not sure if the adaptores are disturbing the normalization-step and if this procedure could influence the sequencing-quality maybe because of some reagents.
(i want to compare sequencing-runs and discover transcripts that are only present in one sample and not in the other. so i do not care about counting transcripts)

thanks. triticum.

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09-15-2009, 12:56 AM	#1
Triticum Member Location: Austria Join Date: Sep 2009 Posts: 12	cDNA normalization hi! is it possible to do a cDNA-normalization prior to the sequencing with solid? the solid-protocol does not mention normalization, but i think it would be very useful for me. i´m not sure if the adaptores are disturbing the normalization-step and if this procedure could influence the sequencing-quality maybe because of some reagents. (i want to compare sequencing-runs and discover transcripts that are only present in one sample and not in the other. so i do not care about counting transcripts) thanks. triticum.