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Old 07-03-2013, 11:50 AM   #1
ein_io
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Location: Bay Area

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Default which colors correspond to which bases

Hi

I am trying to run an Illumina MiSeq reaction in a tube. Does anybody know which dyes correspond to each particular (A, T, G , C) reversible terminator.

Did anybody try to run the reaction in a tube/PCR machine. Any advice on a reference where the conditions (cycle duration, temperature) are mentioned would be highly appreciated.

Thanks in advance!!
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