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Old 12-27-2017, 02:50 AM   #1
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Location: Singapore

Join Date: May 2016
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Default Insert Size in IGV ?


I am visualizing alignments using IGV. I want to ask what do you mean by Insert? Is insert size a large unsequenced segment sitting right in between the two reads which escapes sequencing and also shearing during library prep stage? How to validate the existence of a large insert such as 3Mb or 17 Mb as it cannot be confirmed even by a high fidelity Taq (max limit of Genomic DNA PCR is 1-2kb for taq and upto 30kb for hf Taq)?

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