Hi, I have some duplication issues as suggest in fastQC. The duplication levels in my samples average about 60%. I read some old posts. The link below seems to sugested removal of duplicated reads, while the sequencing facility suggested otherwise. It seems to me that the duplication will affect the accurate counts of the transcripts. Any thoughts?
What software can do this duplication removal? I check out the fastX, it doesn't seem have that functionality. Suggestions?
thanks!
What software can do this duplication removal? I check out the fastX, it doesn't seem have that functionality. Suggestions?
thanks!
Originally posted by GenoMax
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