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Old 03-03-2015, 07:31 AM   #1
Location: Missouri

Join Date: Jan 2013
Posts: 21
Default 2nd Pass Alignment with STAR for RNA-sequencing

Hello everyone,

Firstly, thanks for reading this post. I'm performing 2nd pass alignment with STAR and I was wondering how to merge files. I'll be performing the first phase alignment on ~21 files and will need to merge 21 files. I couldn't find an explanation in the manual on how to merge these files prior to creating a new genome index for the second pass.

I appreciate your time,
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Old 03-03-2015, 07:35 AM   #2
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I'm also having trouble with this concept.

Last edited by mmackattack; 03-03-2015 at 07:43 AM.
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Old 03-03-2015, 09:44 PM   #3
Location: Denmark

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Hopefully your directory structure is setup in such a way, that you can do something along the lines of:

cat /path/to/my/project/results/alignments/mySample*/ >/path/to/desired/output/directory/

Where the asterix could be e.g.: 01,02,...,21

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Old 03-04-2015, 03:59 AM   #4
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Many thanks, Leon.
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alignment, rna-sequencing,, star

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