Hi All,
Our Illumina FAS mentioned that in underloaded lanes on the patterned flow-cells even PCR-free libraries can generate significant numbers of duplicates (clusters swapping over the "walls" into the next empty nanowell if given enough time?).
http://dnatech.genomecenter.ucdavis....data-download/
Would you know of any software that can analyze/remove duplicates based on the coordinate information in the read headers (without any alignments)?
Thanks in advance,
Lutz
Our Illumina FAS mentioned that in underloaded lanes on the patterned flow-cells even PCR-free libraries can generate significant numbers of duplicates (clusters swapping over the "walls" into the next empty nanowell if given enough time?).
http://dnatech.genomecenter.ucdavis....data-download/
Would you know of any software that can analyze/remove duplicates based on the coordinate information in the read headers (without any alignments)?
Thanks in advance,
Lutz
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