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I seem to recall a script for calculating the average distribution of tags per gene from a ChIP-seq experiment. I thought this was a BEDTools script, but don't see it in there. I also couldn't find it in SAMTools. Does anyone know of such a publically available script?
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Maybe coverageBEd in bedtools gets closer to what you want?Originally posted by Giles View Post
DarioCode:coverageBed -abam myreads.bam -b mygenes.gft -hist
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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