SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Nextera Miseq custom primers adziulko Illumina/Solexa 1 05-19-2017 09:31 PM
fusion primers for Illumina Miseq john eimes Sample Prep / Library Generation 4 05-07-2015 11:47 AM
Difference between different Illumina Primers (Nextera, PE etc) ImmuneSeq Illumina/Solexa 1 10-14-2014 03:38 AM
Illumina ITS fusion primers for fungi dr7ski Sample Prep / Library Generation 4 12-02-2013 08:17 AM
Nextera primers wingtec Illumina/Solexa 0 01-31-2013 01:43 PM

Reply
 
Thread Tools
Old 01-25-2018, 08:17 AM   #1
Vasco
Junior Member
 
Location: Germany, Bochum

Join Date: Jan 2015
Posts: 4
Default Illumina fusion primers - Nextera or TruSeq based?

Dear all,

We have been using (old version) TruSeq fusion primers (Illumina flow cell bind, + sequencing primer bind + inline tag + amplification primer) for a while and would now like to add indexing to the P5 and P7 to pool more samples on the same run (Elbrecht Leese in the pictures). When looking into this, we discovered that:

1) We are using a wrong sequences on the P7 (Rd1 SP)
2) When looking at published fusion primer system, mostly TruSeq and Nextera are used (see pictures). But what really confuses us, it that the sequences in the nextera and truseq bases systems are very different! How can this be?

This is really confusion, because I always thought there is one standard index read set included with illumina sequencers, but the different sequences would indicate that for one system custom sequencing primers would have to be added? Am I missing something here?

We would like to have a ready to load fusion primer system, that uses indexing from illumina without the need to provide custom sequencing primers / and that works on all illumina systems (especially MiSeq and HiSeq rapid run). Any advide which (TruSeq or Nexters) to choose here?

What are the advantages of each when used as a one step fusion primer? Both seems to be used in the literature. OR to ask the question differently, what are the actual standard sequencing / indexing primers used, and do they differ between instruments?

Thanks every one = )

Best
Vasco
Attached Images
File Type: jpg DTvI491XUAAwzp-.jpg (71.5 KB, 6 views)
File Type: jpg DTvI492XUAAa4Iz.jpg (84.4 KB, 6 views)
Vasco is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:10 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO