SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
How many number of samples per lane smurugesan Illumina/Solexa 9 08-21-2017 04:02 AM
How indexes are added in TruSeq Custom Amplicon v1.5? Hoatzin Sample Prep / Library Generation 1 01-18-2017 06:40 AM
Illumina's 48 TruSeq Indexes - how many and which really work? skybllue Illumina/Solexa 0 02-20-2014 09:55 AM
TruSeq Library Pooling - Balanced Indexes? acpetterson Illumina/Solexa 6 01-25-2013 04:55 AM
Mixing dual and single index TruSeq samples in a single MiSeq run pmiguel Illumina/Solexa 1 12-21-2012 05:21 AM

Reply
 
Thread Tools
Old 01-30-2018, 01:07 PM   #1
Tamtata
Junior Member
 
Location: SP

Join Date: Jan 2018
Posts: 1
Default TruSeq DNA Single Indexes Sets: How many samples on one lane

Hi

I'm new to Illumina sequencing and would like to run a few tests on a MiSeq platform using a TruSeq DNA Nano kit.

As far as I understand, the Library Prep Kit will allow me to prepare 24 samples, and one of the DNA Single Indexes Sets (A or B) will give me 12 adapters, 24 samples and 48 preps. Does this mean that I can pool 24 samples on one MiSeq lane, and run two lanes? Or am I getting something wrong here? Is there a table with combinations to use for 24 samples on one lane? The library prep guide does not seem to provide this info.

Thanks in advance!
T
Tamtata is offline   Reply With Quote
Old 01-30-2018, 04:30 PM   #2
nucacidhunter
Senior Member
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,171
Default

You can multiplex up to 24 samples/libraries provided that each has different index. For low pooling (less than 6) you need pay attention to index sequences to maximize base diversity (https://support.illumina.com/content...0041074-00.pdf)
nucacidhunter is offline   Reply With Quote
Reply

Tags
illumina, miseq, pooling, trueseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:38 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO