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  • Recommendations for sequencing facility

    Hi
    Can you suggest companies that do RNAseq services? We would like to propose a project and I’d like to get some recommendations for good quality sequencing at a reasonable price.

    thanks

    Charles

  • #2
    Hi Charles,
    I'd be happy to do a quote for you. Although our sequencer is fairly tied up this month, next month we have several RNASeq projects running, so we would try and give you a good price.
    Send me some details of your project and I'll see what we can do for you.
    my email is: [email protected]
    The company website if you want to have a look is www.mgrc.com.my
    Best wishes,
    Jo
    Jo Mason

    [email protected]

    Comment


    • #3
      Hi Charles,

      I'd also be more than happy to write up a quote for you. We use the 454 platform for our cDNA sequencing. My email is [email protected]. Send me a little info about the project and we'll see if we can't work something out.

      The company website is: www.aibiotech.com

      Regards,
      Joe Bertsche

      Comment


      • #4
        What ever you do, DO NOT go with EURO FINS|OPERON !!!!

        Comment


        • #5
          Option

          You may be better of getting it done from some Univ core and some of them also have bionformatics support at nominal charges.

          Comment


          • #6
            try newly organized genome center, global genome research institute, www.ggri.org

            Pricing is one thing. They will consult with you first to figue out the most efficient experimental design and important considerations before contract you.

            Comment


            • #7
              Originally posted by crh View Post
              Hi
              Can you suggest companies that do RNAseq services? We would like to propose a project and I’d like to get some recommendations for good quality sequencing at a reasonable price.

              thanks

              Charles
              Hi Charles,

              I would be happy to provide a quote as well! Please email me at [email protected].

              Lindsay Patty
              DeWalch Technologies
              ls.dewalch.com

              Comment


              • #8
                check out the google Map there are lots of providers on there. I suspect what users want is a way to see who can give back high quality data!

                Comment


                • #9
                  Charles:

                  Expression Analysis (EA) provides high quality RNA Seq services. We are experiencing 110 million reads, consistently, compared to illumina's specs of 60 million reads. We also have an analysis algorythm which enables us to perform alignment and achieve up to 99% alignment. www.expressionanalysis.com

                  Comment


                  • #10
                    We can get as high as 130M reads per lane of HiSeq. However, your target should be more close to 100M passing reads (or not to exceed much beyond 100M) as HiSeq is more sensitive to high density clusters and you will get diminishing return at certain density. Passing rate at high density seems dependent on library type and quality.

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