Hi,
Yesterday i setup a local Silva 123 database for Blastn.
makeblastdb -in LSU.fasta -dbtype nucl -out Silva_LSU
After blastn, i don't know how to analyze the output in MEGAN.
According to the Silva 123 notice, it has MEGAN compatible files as in the follwing website.
tax_TAXNAME_[ls]su_VERSION.{map,tre}
tax_TAXNAME_[ls]su_VERSION.acc_taxid
Then, i download these three files, but i still don't know how to use them?
Which is for "Load RefSeq Mapping file" "Load Gi Mapping File" and "Load Synonyms Mapping File"? Pleas help me.
Yesterday i setup a local Silva 123 database for Blastn.
makeblastdb -in LSU.fasta -dbtype nucl -out Silva_LSU
After blastn, i don't know how to analyze the output in MEGAN.
According to the Silva 123 notice, it has MEGAN compatible files as in the follwing website.
tax_TAXNAME_[ls]su_VERSION.{map,tre}
tax_TAXNAME_[ls]su_VERSION.acc_taxid
Then, i download these three files, but i still don't know how to use them?
Which is for "Load RefSeq Mapping file" "Load Gi Mapping File" and "Load Synonyms Mapping File"? Pleas help me.