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  • How to use Megan analyzing Blastn Silva output?

    Hi,

    Yesterday i setup a local Silva 123 database for Blastn.

    makeblastdb -in LSU.fasta -dbtype nucl -out Silva_LSU

    After blastn, i don't know how to analyze the output in MEGAN.

    According to the Silva 123 notice, it has MEGAN compatible files as in the follwing website.



    tax_TAXNAME_[ls]su_VERSION.{map,tre}
    tax_TAXNAME_[ls]su_VERSION.acc_taxid

    Then, i download these three files, but i still don't know how to use them?
    Which is for "Load RefSeq Mapping file" "Load Gi Mapping File" and "Load Synonyms Mapping File"? Pleas help me.

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