Hi All-
I am generating PCR product of DNA sampled from water (environmental DNA). The amplicons range in size from 650-790 bp. This is the input DNA I would like to use for the Nextera XT sample prep kit from Illumina. I have asked their tech support with out much help on this topic...
If I have a nice clean and tight PCR band on a gel, is there any reason why one can not use exo and sap to "clean" the PCRs before diluting the DNA to 1 ng/uL?
I have over 600 PCRs and doing gel extraction or even a magnetic bead clean up is too much time and money.
Thanks!!
alpinedna
I am generating PCR product of DNA sampled from water (environmental DNA). The amplicons range in size from 650-790 bp. This is the input DNA I would like to use for the Nextera XT sample prep kit from Illumina. I have asked their tech support with out much help on this topic...
If I have a nice clean and tight PCR band on a gel, is there any reason why one can not use exo and sap to "clean" the PCRs before diluting the DNA to 1 ng/uL?
I have over 600 PCRs and doing gel extraction or even a magnetic bead clean up is too much time and money.
Thanks!!
alpinedna
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