Hi all,
I am planning a bacterial genome sequencing project (5 Mb) for around 80 samples to get good coverage. As the input material is limited, I need to use the Nextera XT kit (or standard Nextera kit might work too if we get 50 ng). However, I also want to use the MiSeq v3 kit for the better quality and longer 2 X 300bp reads (25 M reads). Nextera XT size distribution of 300 bp means I'll run into the flowcell and waste read length.
Did anybody use this combination already (Nextera XT + Miseq v3 PE 300 bp) and did you perform a size selection step for frags 500 bp and higher ? Any other suggestion ?
thanks a lot !
Greg
I am planning a bacterial genome sequencing project (5 Mb) for around 80 samples to get good coverage. As the input material is limited, I need to use the Nextera XT kit (or standard Nextera kit might work too if we get 50 ng). However, I also want to use the MiSeq v3 kit for the better quality and longer 2 X 300bp reads (25 M reads). Nextera XT size distribution of 300 bp means I'll run into the flowcell and waste read length.
Did anybody use this combination already (Nextera XT + Miseq v3 PE 300 bp) and did you perform a size selection step for frags 500 bp and higher ? Any other suggestion ?
thanks a lot !
Greg
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