I am new to the field of Library Preparation and RNA-Seq in general, and have some questions about the best options for performing transcriptome analyses on my samples.
I have limited amounts RNA (50-80ng total per sample, at 2-4ng/uL) prepared from my samples of interest, and I am looking to prepare Libraries for sequencing with the NextSeq 500 sequencer.
As far as I can tell, my RNA quantities are too low for traditional library preparation, leaving me with a choice between RNA amplification kits or alternative library preparation kits such as the TruSeq RNA Access Kit (which isolates RNA coding regions for sequencing). Is there a clear advantage to choosing one of these strategies over the other? Are there alternative strategies/kits that I am not aware of that might work bettter? I would greatly appreciate any help that you can provide.
I have limited amounts RNA (50-80ng total per sample, at 2-4ng/uL) prepared from my samples of interest, and I am looking to prepare Libraries for sequencing with the NextSeq 500 sequencer.
As far as I can tell, my RNA quantities are too low for traditional library preparation, leaving me with a choice between RNA amplification kits or alternative library preparation kits such as the TruSeq RNA Access Kit (which isolates RNA coding regions for sequencing). Is there a clear advantage to choosing one of these strategies over the other? Are there alternative strategies/kits that I am not aware of that might work bettter? I would greatly appreciate any help that you can provide.
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