SEQC in Nature Web Collection

[plabaj]

This Web Collection presents the results of the RNA Sequencing Quality Control (SEQC) project that sought to evaluate the reproducibility and comparability of high-throughput sequencing of RNA (RNA-seq). Data from several different laboratories are compared and the performance of different sequencing platforms and data analysis approaches are assessed, together with benchmarking against DNA microarrays. Ultimately, these multi-platform, cross-site studies will enable RNA-seq to be applied more broadly in analyzing large cohorts for discovery research and clinical use. This is the latest phase of a collaboration between government, academic and industry researchers as part of the MicroArray Quality Control (MAQC) consortium.

http://www.nature.com/nbt/collections/seqc/index.html

[plabaj]

Data sets from this study are going to be used in CAMDA 2015 challenges (www.camda.info):

For 2015, the FDA SEQC consortium offers two challenges:
(i) the response of over 100 rat livers to 27 drugs representing 9 different chemical modes of action, as characterized by matched RNA-Seq and microarray expression profiles, and
(ii) a multi-site cross-platform RNA-Seq benchmark covering 3 next-generation sequencing, 5 microarray, and 2 qPCR platforms.