Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • RNA-seq from oligo(dT) primed cDNA

    A collaborator has a good set of oligo(dT) primed cDNAs. Does anybody have an opinion if it would be reasonable to use these for RNA-seq or would it be a waste of time and money?

    The sample collection is not trivial, will take 6 to 12 months and involves recruiting patients and subjecting them to a somewhat uncomfortable procedure.
    --------------
    Ethan

  • #2
    Originally posted by ETHANol View Post
    A collaborator has a good set of oligo(dT) primed cDNAs. Does anybody have an opinion if it would be reasonable to use these for RNA-seq or would it be a waste of time and money?
    In principle, nothing wrong with this approach. Depends what you mean by "good". Are there adapters on these cDNAs? What average length are they? Are they double stranded? Were they ribo-depleted?

    Originally posted by ETHANol View Post
    The sample collection is not trivial, will take 6 to 12 months and involves recruiting patients and subjecting them to a somewhat uncomfortable procedure.
    You mean if they have to be repeated, right?

    --
    Phillip

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    66 views
    0 likes
    Last Post seqadmin  
    Working...
    X