Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • get mate pair info from xml file

    Hello i'm trying to assembly a sequencing done 100% with sanger reads, using celera assembler

    i have the fasta, fasta.qual, anc, clip and xml files from NCBI Trace

    the xml files have the mate pair info, but when i run the assembler, skip this info

    from the final .qc file

    [Mates]
    ReadsWithNoMate=691348(100.00%)
    ReadsWithGoodMate=0(0.00%)
    ReadsWithBadShortMate=0(0.00%)
    ReadsWithBadLongMate=0(0.00%)
    ReadsWithSameOrientMate=0(0.00%)
    ReadsWithOuttieMate=0(0.00%)
    ReadsWithBothChaffMate=0(0.00%)
    ReadsWithChaffMate=0(0.00%)
    ReadsWithBothDegenMate=0(0.00%)
    ReadsWithDegenMate=0(0.00%)
    ReadsWithBothSurrMate=0(0.00%)
    ReadsWithSurrogateMate=0(0.00%)
    ReadsWithDiffScafMate=0(0.00%)
    ReadsWithUnassignedMate=0(0.00%)
    TotalScaffoldLinks=0
    MeanScaffoldLinkWeight=0.00



    the xml file looks like


    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>690</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>PACH2306</TEMPLATE_ID>
    <TI>451441523</TI>
    <TRACE_DIRECTION>FORWARD</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>PACH2306.x1</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>
    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>933</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>PACH2306</TEMPLATE_ID>
    <TI>451443045</TI>
    <TRACE_DIRECTION>REVERSE</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>PACH2306.y1</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>
    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>888</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>ACBG121468</TEMPLATE_ID>
    <TI>452858124</TI>
    <TRACE_DIRECTION>FORWARD</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>ACBG121468.b2</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>
    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>872</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>ACBG121468</TEMPLATE_ID>
    <TI>452859852</TI>
    <TRACE_DIRECTION>REVERSE</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>ACBG121468.g2</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>



    i need to extract this info in some way, and pass to celera assembler??

    or i need a special flag so celera assembler use this??

    thx in advance

    Cristian

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
55 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
52 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
45 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X