Hi,
I'm new to Illumina sequencing. Is there any guideline for size selection step in terms of how many bp are the most often used in Illumina pair end sequencing? 200-300bp?
Will this step bring in bias because fragments of other length are eliminated?
I plan to use NEBnext Ultra DNA library prep kit. Any tricks or suggestions about using this kit and AMPure XP beads for size selection?
Thanks a lot
I'm new to Illumina sequencing. Is there any guideline for size selection step in terms of how many bp are the most often used in Illumina pair end sequencing? 200-300bp?
Will this step bring in bias because fragments of other length are eliminated?
I plan to use NEBnext Ultra DNA library prep kit. Any tricks or suggestions about using this kit and AMPure XP beads for size selection?
Thanks a lot
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