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Old 06-23-2009, 05:00 AM   #1
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Default PubMed: Filter-based hybridization capture of subgenomes enables resequencing and cop

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Related Articles Filter-based hybridization capture of subgenomes enables resequencing and copy-number detection.

Nat Methods. 2009 Jun 21;

Authors: Herman DS, Hovingh GK, Iartchouk O, Rehm HL, Kucherlapati R, Seidman JG, Seidman CE

To exploit contemporary sequencing technologies for targeted genetic analyses, we developed a hybridization enrichment strategy for DNA capture that uses PCR products as subgenomic traps. We applied this strategy to 115 kilobases of the human genome encompassing 47 genes implicated in cardiovascular disease. Massively parallel sequencing of captured subgenomic libraries interrogated 99.8% of targeted nucleotides >/=20 times ( approximately 40,000-fold enrichment), enabling sensitive and specific detection of sequence variation and copy-number variation.

PMID: 19543287 [PubMed - as supplied by publisher]

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