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Old 05-09-2013, 09:38 AM   #1
alphy
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Location: Buffalo, ny

Join Date: Oct 2011
Posts: 1
Default TruSeq ChIP sample Prep - size selection using Blue pippen

Has anyone used Blue Pippen to do size selection after adapter ligation. I want to know what size range do you choose. I have done the size selection after the PCR amplification but I want to try to do the size selection before the PCR reaction.
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Old 05-22-2013, 06:48 AM   #2
Chris Boles
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Location: Boston

Join Date: Aug 2009
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Default Pippin Prep help on ChIP-seq applications

Dear Alphy,

I would be happy to try and find a Pippin Prep customer with experience in ChIP-seq, who might be able to help you with those settings. Please send a private message to me (via my SEQanswers profile) with your email address. (I don't want to publish private customer email addresses here.)

Our customers who perform size selection on Truseq libraries prior to amplification enrichment, usually find that they have to use size selection settings significantly larger than the desired insert size (settings 130-150 bp larger than the desired insert sizes, for inserts in the 400-500 bp range).

Chris Boles, Sage Science
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